William Shakespeare and His Works Essay Example for Free

William Shakespeare and His Works Essay William Shakespeare was born on April 23, 1564 in the home of John Shakespeare and Mary Arden at Stratford-on-Avon. He was educated at the King Edward IV Grammar School in Stratford, where he learned Latin and a little Greek and read the Roman dramatists. At eighteen, he married Anne Hathaway, a woman seven or eight years his senior. Together they raised Susanna, who was born in 1583, and the twins Judith and Hamnet (who died in boyhood), born in 1585. He was an English poet and playwright, widely regarded as the greatest writer in the English language and the worlds pre-eminent dramatist. He is often called Englands national poet and the Bard of Avon. Between 1585 and 1592, he began a successful career in London as an actor, writer, and part owner of a playing company called the Lord Chamberlains Men, later known as the Kings Men. In 1594, Shakespeare joined the Lord Chamberlains company of actors, the most popular of the companies acting at Court. The Globe, which became the most famous theater of its time. With his share of the income from the Globe, Shakespeare was able to purchase New Place, his home in Stratford. Shakespeare produced most of his known work between 1589 and 1613. His early plays were mainly comedies and histories, genres he raised to the peak of sophistication and artistry by the end of the 16th century. He then wrote mainly tragedies until about 1608, including Hamlet, King Lear, Othello, and Macbeth, considered some of the finest works in the English language. In his last phase, he wrote tragicomedies, also known as romances, and collaborated with other playwrights. In 1623, John Heminges and Henry Condell, two friends and fellow actors of Shakespeare, published the First Folio, a collected edition of his dramatic works that included all but two of the plays now recognised as Shakespeares. Shakespeare was a respected poet and playwright in his own day, but his reputation did not rise to its present heights until the 19th century. The Romantics, in particular, acclaimed Shakespeares genius, and the Victorians worshipped Shakespeare. In the 20th century, his work was repeatedly adopted and rediscovered by new movements in scholarship and performance. His plays remain highly popular today and are constantly studied, performed, and reinterpreted in diverse cultural and political contexts throughout the world. In his poems and plays, Shakespeare invented thousands of words, often combining or contorting Latin, French and native roots. His impressive expansion of the English language, according to the Oxford English Dictionary, includes such words as: arch-villain, birthplace, bloodsucking, courtship, dewdrop, downstairs, fanged, heartsore, hunchbacked, leapfrog, misquote, pageantry, radiance, schoolboy, stillborn, watchdog, and zany. His extant works, including some collaborations, consist of about 38 plays, 154 sonnets, two long narrative poems, and a few other verses. Only eighteen of Shakespeares plays were published separately in quarto editions during his lifetime. He retired to Stratford around 1613 at age 49, where he died three years later. Superstitions during Shakespearean time Superstitions are irrational beliefs but a handful of them are actually still evident in our modern world. The superstitions that originated during the Elizabethan era were based on various beliefs and traditions. The historians opine that many of the traditional English customs were based on the myths and superstitions that date back to the Dark Ages. Ignorance and fear of the unknown, combined with a false conception of death resulted in many superstitions during the Elizabethan era. Shakespeare had made use of the superstitions regarding spirits and witchcraft that prevailed in the Elizabethan society in his plays Macbeth and Hamlet. Books by Shakespeare Romeo and Juliet The play begins with a large fight between the Capulet’s and the Montague’s, two prestigious families in Verona, Italy. Meanwhile, Romeo and Benvolio are accidentally invited to their enemy’s party. At the party, Romeo locks eyes with a young woman named Juliet. They instantly fall in love, but they do not realize that their families are mortal enemies. When they realize each other’s identities, they are devastated, Romeo sneaks into Juliet’s yard after the party and proclaims his love for her. She returns his sentiments and the two decide to marry. The next day, Romeo and Juliet are married Juliet’s mother, informs Juliet that she will marry a man named Paris in a few days. Juliet asks Friar Lawrence for advice. Friar Lawrence gives Juliet a potion which will make her appear dead and tells her to take it the night before the wedding. Juliet drinks the potion and everybody assumes that she is dead. Romeo assumes that his wife is dead. He rushes to Juliet’s tomb and, in deep grief, drinks a vial of poison. later, Juliet wakes to find Romeo dead and kills herself due to grief. Once the families discover what happened, they gather sufficient self-knowledge to correct their. Macbeth It is considered one of Shakespeare’s darkest and most powerful tragedies. Set in Scotland when its protagonist, the Scottish lord Macbeth, chooses evil as the way to fulfill his ambition for power. The play is believed to have been written between 1603 and 1607, Macbeth receives a prophecy from a trio of witches that one day he will become King of Scotland. Consumed by ambition and spurred to action by his wife, Macbeth murders King Duncan and takes the throne for himself. He is then forced to commit more and more murders to protect himself from enmity and suspicion. The bloodbath swiftly takes Macbeth and Lady Macbeth into the realms of arrogance, madness, and death. Othello Believed to have been written in approximately 1603, and based on the Italian short story Un Capuano Moro (A Moorish Captain) by Cinthio. The work revolves around four central characters: Othello, a Moorish general in the Venetian army; his wife, Desdemona; his lieutenant, Cassio; and his trusted ensign, Iago. Because of its varied and current themes of racism, love, jealousy, and betrayal, Othello is still often performed in professional and community theatres and has been the basis for numerous operatic, film, and literary adaptations. The Tempest Believed to have been written in 1610–11. The Tempest attained popularity only after the Restoration. It is set on a remote island, where Prospero, the rightful Duke of Milan, plots to restore his daughter Miranda He conjures up a storm, the eponymous tempest, to lure his usurping brother Antonio and the complicit King Alonso of Naples to the island. There, his machinations bring about the marriage of Miranda to Alonsos son, Ferdinand. The story draws heavily on the tradition of the romance, and it was influenced by tragicomedy and the courtly masque. It differs from Shakespeares other plays in its observation of a stricter, more organized neoclassical style. Twelfth Night Twelfth Night or, what you will is a comedy by William Shakespeare, believed to have been written around 1601–02 for the close of the Christmas season. The play expanded on the musical interludes and riotous disorder expected of the occasion Much Ado about Nothing Much Ado About Nothing is a comedic play by William Shakespeare thought to have been written in 1598 and 1599, as Shakespeare was approaching the middle of his Much Ado About Nothing chronicles two pairs of lovers: Benedick and Beatrice (the main couple), and Claudio and Hero (the secondary couple). Benedick and Beatrice are engaged in a very merry war; they are both very witty and proclaim their disdain of love. In contrast, Claudio and Hero are sweet young people who are rendered practically speechless by their love for one another career. The courtship between the wittier, wiser lovers Benedict and Beatrice is what makes Much Ado about Nothing so memorable. Benedick and Beatrice argue with delightful wit, and Shakespeare develops their journey from antagonism to sincere love and affection with a rich sense of humor and compassion. Benedick and Beatrice are tricked into confessing their love for each other. Dogberry, a Constable who is a master of malapropisms, discovers the evil trickery of the villain, Don John. In the end, Don John runs away and everyone else joins in a dance celebrating the marriages of the two couples. As You Like It It is a pastoral comedy by William Shakespeare believed to have been written in 1599 or early 1600 . The play features one of Shakespeares most famous and oft-quoted speeches The play remains a favorite among audiences and has been adapted for radio, film, and musical theatre. As You Like It follows its heroine Rosalind as she flees persecution in her uncles court, accompanied by her cousin Celia and Touchstone the court jester, to find safety and eventually, love, in the Forest of Arden. Julius Caesar Julius Caesar is a tragedy believed to have been written in 1599. It portrays the conspiracy against the Roman dictator Julius Caesar, his assassination and the defeat of the conspirators at the Battle of Philippi. It is one of several plays written by Shakespeare based on true events from Roman  history,. Although the title is Julius Caesar, Julius Caesar is not the most visible character in its action; and is killed at the beginning. The central psychological drama is Marcus Brutus’s struggle between the conflicting demands of honor, patriotism, and friendship. The Comedy of Errors The Comedy of Errors is one of William Shakespeares earliest plays. It is his shortest and one of his most farcical comedies, with a major part of the humor coming from slapstick and mistaken identity. The Comedy of Errors tells the story of two sets of identical twins that were accidentally separated at birth. Antipholus of Syracuse and his servant, Dromio of Syracuse, arrive in Ephesus, which turns out to be the home of the twin brothers, When the Syracusans encounter the friends and families of their twins, a series of wild mishaps based on mistaken identities lead to wrongful beatings, a near-seduction, the arrest of Antipholus of Ephesus, and false accusations of infidelity, theft, madness, and demonic possession.

Overview Of Vowels And Consonants

Overview Of Vowels And Consonants Language is an organization of sounds, of vocal symbols-the sounds produced from the mouth with the help of various organs of speech to convey some meaningful message. Language has a very important social purpose, because it is mainly used for linguistic communication. It is the most powerful, convenient and permanent means and form of communication. A language can be used in two ways for the purposes of communication. It can be spoken or written but the medium of speech is more important than writing. This is because speech comes first in the history of any language community in fact, it came centuries before writing in the history of any language community. Secondly, speech comes first in the history of any individual. We started speaking long before we started writing. Speech as a medium of communication is used much more than the medium of writing. I n every language, a letter of the alphabet represents a particular sound. Lastly, modern technology has contributed tremendously t o the importance of speech- modern inventions like the telephone, the radio, the tape recorder and several such devices have raised problems of communication primarily concerned with speech. Linguistics is a systematic study of language. Phonetics is a branch of linguistics and it is the branch dealing with the medium of speech. It deals with the production, transmission and reception of the sounds of human speech. For the production of speech sounds, we need an air-stream mechanism. There are three main air-stream mechanisms, such as, pulmonic, glottalic and velaric air-stream mechanisms. When the air-stream mechanism is used to push out, it is called egressive and when it is used to draw air in, it is called ingressive. Most sounds of most languages in the world are produced with a pulmonic egressive air-stream mechanism. The author described in detail the various organs that are responsible for converting the lung-air into speech sounds before it escapes into the outer atmosphere. For instance, if we say a prolonged ssss, a prolonged zzzz, a prolonged ffff and a prolonged vvvv, we see at once two things. We recognize these as speech sounds because these sounds occur i n the various words we use in our English speech. The other thing we notice is that each one of these sounds is different from the others. Speech sounds are very broadly divided into two categories, namely, Vowels and Consonants. If we say the English word shoe, we realize that this word is made up of two sounds, one represented by the letters sh and the other represented by the letters oe. When we produce the sound represented by the letters sh slowly, we realize that during the production of this sound, the air escapes through the mouth with friction. On the other hand, when we produce the sound represented by the letters oe, the air escapes through the mouth freely and we do not hear any friction. The sound is represented by the letters sh in the word shoe is a consonant and the sound represented by the letters oe in the word shoe is a vowel. All sounds during the production of which we hear friction are consonants, but not all consonants are produced with friction. If we say the words, she, shoe, shy, show, ship and shout, we will realize that when we produce the sounds represented by the letters e, oe, y, ow, i and ou in these words, the air escapes through the mouth freely without any friction. All these sounds are therefore vowels but each one of them sounds different from the others. These sounds should therefore be sub-classified. Similarly, if we say the words shoe, see, zoo and who, we will hear friction during the production of the sounds represented by the letters sh, s, z and wh. All these sounds are therefore consonants. But once again, we will see that each of them sounds different from the others. The sounds that are called consonants also need to be sub-classified. Description of Consonants: The word consonant has been derived from the Greek word consonautem, which means the sound produced with the help of some other sound (vowel). A consonant is usually described, taking into account whether it is voiceless or voiced, its place of articulation and its manner of articulation. Manner of articulation refers to the stricture involved and plosive, affricate, nasal, fricative, etc; are labels given to consonants according to their manner of articulation. Place of articulation just means the two articulators involved in the production of a consonant. Consonants can be described according to their places of articulation. The label used is normally an adjective derived from the name of the passive articulator. The places of articulation that we frequently come across are bilabial, labio-dental, dental, alveolar, post-alveolar, palato-alveolar, retroflex, palatal, velar, uvular and glottal. The classification of sounds into vowels and consonants is customary irrespective of phonetic, phonological, or orthographic references. The current classification following Pike divides the sounds into vocoids (vowel sounds), contoids (consonant sounds) and semi-vocoids or semi-contoids (for example; /w/ and /j/ in English). The terms contoids and vocoids refer to phonetic form only, without any reference to phonological function. A vocoid, according to Pike, is a segment formed with an open approximation of the articulators, with or without a velic closure, and with central passage or air-stream. All other segments are contoids. In English, there can be syllabic vocoids, non-syllabic vocoids, syllabic contoids and non-syllabic contoids. Syllabic vocoids are all vowel sounds; they function as syllable nuclei. Phonetically, the vocoids are vowels and their phonological function is that of a syllabic vocoid. Non-syllabic vocoids are the sounds which are phonetically vocoids but phonologically are contoids. Syllabic contoids are the sounds which are phonetically contoids but their phonological function is that of syllabic nucleus, that is, they represent the V element in the structure of a syllable. Non-syllabic contoids are the sounds that phonetically are contoids and phonologically represent the C element in the structure of a syllable. A description of consonantal sounds, according to A.C.Gimson, must provide answers to the following questions:- Is the air-stream set in motion by the lungs or by some other means (pulmonic or non-pulmonic)? Is the air-stream forced outwards or sucked inwards (egressive or ingressive)? Do the vocal cords vibrate or not (voiced or voiceless)? Is the soft palate raised or lowered? Or, does the air pass through the oral cavity (mouth) or the nasal cavity (nose)? At what point or points and between what organs does the closure or narrowing take place (Place of articulation)? What is the type of closure or narrowing at the point of articulation (Manner of articulation)? Thus, the description of a consonant will include five kinds of information: 1.) The nature of air-stream mechanism, 2.) The state of the glottis, 3.) The position of the soft palate, 4.) The articulators involved- the active articulator and the passive articulator and 5.) The nature of stricture involved regarding its production. The Nature of Air-Stream Mechanism: All English sounds, vowels as well as consonants, are produced with a Pulmonic egressive air-stream mechanism, that is, the lung-air pushed out. The State of the Glottis: Speech sounds can be classified as voiceless or voiced, depending upon whether the vocal cords are wide apart and the glottis is wide open (voiceless) or the vocal cords are kept loosely together and they vibrate (voiced). The Position of the Soft Palate: Speech sounds can be classified as oral or nasal, depending upon whether the soft-palate is raised so as to shut off the nasal passage of air (oral) or it is lowered to open the nasal passage of air simultaneously with an oral closure (nasal). Sounds can also be nasalised. The Articulators Involved-the Active and Passive Articulators: Of the various articulators described in the chapter, at least two are required for the production of any speech sound; some articulators move during the production of speech sounds. These are termed as active articulators. Certain other articulators remain passive and the active articulators move in the direction of these. These are termed as passive articulators. The Nature of Stricture Involved: The term stricture refers to the way in which the passage of air is restricted by the various organs of speech. The stricture may be one of complete closure, that is, the active and passive articulators come into firm contact with each other, thus preventing the lung-air from escaping through the mouth. Simultaneously, there is a velic closure, that is, the soft palate is raised, thereby shutting off the nasal passage of air. Thus, the lung-air is blocked in the mouth. When the oral closure is released, that is, when the active articulator is suddenly removed from the passive articulator, the air escapes with a small explosive noise. Sounds produced with a stricture of complete closure and sudden release are called plosives. If the active articulator is removed slowly from the passive articulator, instead of the explosive noise that is characteristic of plosive consonants, friction will be heard. Description of Vowels: Vowels may be defined with an open approximation without any obstruction, partial or complete, in the air passage. They are referred to as vocoids in phonetics. They can be described in terms of three variables: Height of tongue. Part of the tongue which is raised or lowered. Lip-rounding. So vocoids are normally classified according to these three criteria: tongue-height (high, mid, low, or close, half-close, half-open and open), tongue-advancement (front, central, back) and lip-rounding (rounded and unrounded). In order to describe the vowels, we usually draw three points in the horizontal axes: front, central and back, referring to the part of the tongue which is the highest. So, we have : Front vowels, during the production of which the front of the tongue is raised towards the hard palate. For example; / i, i: , e: , a / in Hindi, and / i, i: , e , à ¦ / in English as in sit, seat, set, and sat respectively. Back vowels, during the production of which the back of the tongue is raised towards the soft palate. For example; / o: , u , u: ,/ in Hindi, and / a: , Ɇ , ɆÃƒâ€¹Ã‚  , u , u: / in English as in cart, cot, caught, book and tool respectively. Central vowels, during the production of which the central part of the tongue ( the part between the front and the back) is raised. For example; / Éâ„ ¢ / in Hindi, and / Éâ„ ¢ , Éâ„ ¢: , ÊŒ / in English as in about, earth and but respectively. O n the vertical axis, we usually draw four points: close, half-close, half-open, and open. They are also referred to as high, high mid, mid (middle), low mid, and low by some phoneticians, especially the American phoneticians. On the basis of the vertical axes, we have the following types of vowels. A close vowel is one for which the tongue is as close to the roof of mouth as possible. For example; / i: / in sea and / u: / in zoo. An open vowel is one which is produced with the tongue as low as possible and the jaws are wide open. For example, / a: / in card and / Ɇ / in hot. We can describe a vowel by using a three term label, indicating the height, the direction (advancement) of the tongue, and the position of the lips. For example; / a: / in the English word, arm, back, open, unrounded vowel. / Ɇ / in the English word, hot, back, open, rounded vowel. / i: / in the English word, need, front, close, unrounded vowel. / u / in the English word, tooth, back, close, rounded vowel. To describe the vowel sound, we mention whether it is open or close, half-close or half-open, front or back or central, long or short, whether the tongue is tense or lax while the vowel is being pronounced, and whether lips are spread, neutral, open rounded, or close rounded. All English vowels are voiced. So, for every vowel, we must state that it is voiced. Hence, to sum up, the main point of a language is to convey information. Nowadays, language can take various forms. It can be spoken or written. Peter Ladefoged also talked in his book that speech is the common way of using language. Another aspect of speech that is not part of language is the way speech conveys information about the speakers attitude to life, the subject under discussion and the person spoken to. The final kind of non-linguistic information conveyed by speech is the identity of the speaker. You can often tell the identity of the person who is speaking without looking at them. But then again, we may be wrong. Whenever we speak, we create a disturbance in the air around us, a sound wave, which is a small but rapid variation in air pressure spreading through the air. Speech sounds such as vowels can differ in pitch, loudness and quality. We can say the vowel a as in father on any pitch within the range of our voice. We can also say it softly or loudly without altering the pitch. And we can say as many different vowels as we can, without altering either the pitch or the loudness. The pitch of the sound depends on the rate of repetition of the changes in air pressure. The loudness of the sound depends on the size of the variations in air pressure. The third way in which sounds can differ is in quality, sometimes called timbre. The vowel in see differs in quality from the first vowel in father., irrespective of whether it also differs in pitch or loudness. Thus, Peter Ladefoged in his book has discussed the principal constraints on the evolution of the sounds of the worlds languages, which are ease of articulation, auditory distinctiveness, and gestural economy. He also discussed the differences between speech and language, and has also outlined some of the main acoustic distinctions among sounds; and how one of the acoustic distinctions, that corresponding to pitch, is used in the worlds languages.

Population Genetics (Molecular Epidemiology) of Eukaryotes

Population Genetics (Molecular Epidemiology) of Eukaryotes INTRODUCTION The study of the molecular epidemiology of parasitic infections and their vectors is meant to answer the same kinds of questions as those of bacterial or viral infections. As with bacteria, the molecular epidemiology of eukaryotic infections follows the distribution and dynamics of microbial DNA. The key difference, however, is precisely this biology, which defines a distinct approach to molecular epidemiologic investigation of infections caused by eukaryotic organisms. In bacterial reproduction, each individual passes down an identical copy of all the DNA to the next generation. Some eukaryotic pathogens behave reproductively in similar ways to bacteria and reproduce asexually, while others have sexual reproduction for at least part of their life-cycle. The individual is able to generate a clone of itself by binary fission to produce two identical organisms, and if successful, will produce large numbers to the detriment of its host. Asexually reproducing organisms can also exhibit p romiscuous horizontal gene transfer, which can be a major source of variation and adaptation (19), but this is not sex. Sex is the biologically necessary programmed recombination (crossing over) and random shuffling (reassortment) of chromosomal DNA in the process of reproduction. This results in an enormous reservoir of variation. Bacteria in nature are heterogenous conglomerates or communities (13, 19), but when they cause disease, especially in epidemics, it is generally a clone that is responsible and that we track (Chapter 2). Sexual reproduction in some protozoa, many parasitic worms and most vectors, however, never results in a clone with the exception of identical twins. There is genetic conservation, however, within a group of organisms that tends to breed together. In genetics, this is the working definition of a population. For sexually reproducing organisms, the population is the epidemiologic unit to track. Within the group, allele frequencies and thus traits are conser ved under well-defined conditions. The unique power of the genetics of populations is that it reflects not only present individuals but also the populations past and the future potential for subsequent generations (5). Many parasites exhibit both sexual and asexual modes of reproduction, but these life stages are distributed in different hosts. Treatment of their molecular epidemiology is doubly complex, but can be simplified for some questions by considering their biology just in the human host. The whole field of population genetics is perhaps the most complex area of genetics, but it arises from simple precepts. This chapter will outline the basic models used in population genetics and are directly applicable to problems of public health epidemiology. KEY POINTS Asexual reproduction usually produces a clone; sexual reproduction never does. A population is a group of organisms that tends to breed together Allele and genotype frequencies describe populations Allele frequencies and traits tend to be maintained within groups of interbreeding organisms (derived from the Hardy-Weinberg equilibrium) Allele and genotype frequencies can be used to infer population histories Indices and statistics can be used to compare assess population history and to project population dynamics DEFINING GENOTYPE IN EUKARYOTIC ORGANISM   Ã‚   Some terms may not be familiar to some readers, so it is important to define these early. One of the dividing lines between bacteria and sexually reproducing parasites and vectors of human disease is their physical structure and organization. Sexually reproducing organisms will pass some portion of their life cycle where their chromosomes (Figure 5.1) exist as nearly identical pairs (diploid). Some organisms, malaria in particular, also have only one copy (haploid) during their asexual stage, and this is the stage that infects humans. A similar location on each of the chormosomes is a locus, and differences between loci are alleles. The geometry of DNA also strongly differentiates bacteria from eukaryotes (Figure 5.2). Prokaryotes have a single[1], circular chromosome whereas even the simplest eukaryotes, yeast, have at least 16 linear chromosomes. A specific marker on a bacterial chromosome will always be transmitted at reproduction together with any other marker or trait. The same also occurs with an asexually reproducing eukaryote despite having multiple linear chromosomes. A marker on the genome of a sexually reproducing eukaryote, by contrast, will have a 50% chance of being transmitted away from any marker it is not very close to. The labeling of each allele present at the same locus on each chromosome constitutes the genotype. A locus with the same polymorphism at the same site on each of the chromosome is homozygous, and with a different polymorphism is heterozygous. Figure 5.2 OPTIONS FOR MOLECULAR EPIDEMIOLOGY OF EUKARYOTES Study asexual parasites Use a marker close to the trait of interest (if known) Use many markers throughout the genome or sequence Study the whole group of organisms in which the trait is present (population) HARDY-WEINBERG EQUILIBRIUM: THE POPULATION NULL HYPOTHESIS Populations have a mathematical definition based on allele frequencies, which ultimately contributes to the development of tools for key measures of differentiation and diversity. Allele frequencies can differentiate populations, and genotypic frequencies can do so with even greater resolution. The relationship between allelic frequency and genotypic frequency has a simple mathematical relationship which is the definition of a population. If we use the letters A and a to represent different alleles at a single diallelic locus and p and q to represent their respective frequencies, a population with p=0.8 and q=0.2 is clearly different from a population where p=0.2, q=0.8, especially where this kind of result is found at multiple loci. Allele frequencies are not always the most sensitive measure of differentiation. The same allele frequency may still be found in what are clearly distinct populations if assessed for genotypic frequencies. Alleles combine to form genotypes, so the genoty pic frequency is a function of the allelic frequencies. For a diallelic locus where we know the frequency of each allele, the sum of these frequencies is 1 or (p + q = 1). For sexually reproducing organisms the next generation arises from the combination of alleles from a pool of males with alleles from a pool of females. If we imagine that individuals from these pools will pair at random, the subsequent distribution of alleles in genotypes is equivalent to rolling a pair of dice. For independent, random events the probability of 2 events occurring simultaneously is the product of their frequencies [(p + q)female à ¢Ã¢â€š ¬Ã‚ ¢ (p + q)male = 1]. The genotypic frequencies of the offspring for such a population should be p2 + 2pq + q2, if all assumptions are met, where p2 and q2 are the frequencies for the homozygotes and 2pq the heterozygotes. This is the well-known Hardy-Weinberg equilibrium (HWE). This simple quadratic equation is the basis for all population genetics even when it is not measured directly. It represents the expected genotypic frequencies from a given set of allelic frequencies. It is one of the most stable mathematical relationships in nature. It is so much the expectation that when not observed in sequencing projects, it can suggest sequencing errors. It is the null hypothesis and mathematical definition of a stable population. The relationship HWE describes is true under a set of 5-10 assumptions that represent the most important factors that influence population genetic structure. The 5 most common assumptions are that there is: 1) Random mating (panmixia, assortative mating) 2) No selection 3) No migration 4) An infinite population 5) No mutation It is rare to have any of these assumptions met in nature, but the proportions are so resilient that the assumptions have to be severely violated to disturb this relationship, and even so, the proportions will be reestablished within 1-2 generations once the population is stabilized. As with most models, the underlying assumptions are the most important aspects. They are the basis for most conversations in population genetics. MARKERS Microsatellites, single nucleotide polymorphisms (SNPs) and sequencing are currently the genetic elements most employed in population genetics. Microsatellites are short tandem repeats of 2-8 nucleotides (reviewed in [Ellegren, 2004 #128]).  Ãƒâ€šÃ‚   Microsatellites have fallen out of favor in studies of statistical genetics or gene finding, since SNPs and sequencing provide better resolution at the level of individuals. Microsatellites, however, remain important in population genetics since they are mostly neutral for selection and have higher allelic richness and information content. Their rapid mutation rate (10-2 à ¢Ã¢â‚¬ Ã¢â€š ¬ 10-5 per generation) and step-wise mode of mutation can limit their application to questions that extend over short time scales and to certain statistical approaches. SNPs have lower rates of mutation (10-8) in eukaryotes, often are diallelic, are ten times more abundant (10, 22) and have high processivity and scorability. Sequencing essentiall y provides a very dense panel of SNPs and identifies rare variants as well as structural polymorphisms. Mitochondrial and ribosomal DNA markers are much less abundant, less polymorphic and thus less informative than microsatellites or SNPs. Some are under selection and in the case of mitochondrial DNA, the genome is haploid (only 1 copy of chromosomal DNA) and may or may not have sex-specific inheritance depending on the species. They are useful for phylogeny studies, may be more economical to use in laboratories with limited capabilities and are sometimes combined with other markers. MEASURES OF DIFFERENTIATION AND DIVERSITY Areas most often addressed using population genetics are evolution and conservation. These two areas deal with essentially the same phenomenon, but at different time scales, thus the questions, the approaches and the interpretation will differ depending on the nature of the problem. The relevant public health questions in population genetics focus on identity and dynamics of the group rather than individuals over short time scales and directed at the control or extinction of a parasite or vector. Whos sleeping with whom, modes of reproduction, evolution or the last common ancestor are all important in different contexts. They may be useful to help explain anomalies and can influence interpretation, but they are rarely answers to issues of control or intervention. Understanding how diverse a population is or the degree of difference between populations combined with good study design will contribute directly to determining the impact of control measures, host or parasite demographics, resistance, risk and resilience or fragility of the population. The field of population genetics depends heavily on mathematical analyses, some simple and some very sophisticated, to answer these questions. Mathematical treatments of all of the indices and statistics of differentiation and diversity can be easily obtained from textbooks or publications and will not be included here. Fortunately for the mathematically challenged, many open source, individual computer programs are available as well as modules in R. The risk that goes with all readymade programs is a failure to understand what is being asked or the assumptions and limitations of the approach being taken. A list of some frequently used programs is provided at the end of this chapter (Table 5.1). POPULATIN DIFFERENTIATION FST, GST, GST: In addition to the Hardy-Weinberg equilibrium, populations can be further differentiated by other statistical tests.   This is a family of statistics developed as the fixation index (FST) in the 1950s by Sewell Wright and Gustave Malà ©cot to describe the likelihood of homozygosity (fixation in the terminology of the time) at a single diallelic locus based on heterozygosity of a subpopulation compared to the total population. Theoretically, values should range from 1 (no similarity-every individual is genotypically different) to 0 (identity-every individual is genotypically same). Nei (16) extended the FST to handle the case of more than two alleles and developed the GST[LR1]. Although the term FST is often used in the literature, formally most studies today will employ the GST. When highly polymorphic loci, such as microsatellites, are genotyped, the GST severely underestimates differentiation and will not range from 0 1. Hedrick (11) adjusted the range of va lues for the GST by dividing it by its maximum possible value given the markers used. This is the GST. The GST makes possible the full range of differentiation. FST and GST relate to inherent properties of populations and contain evolutionary information lost by the GST transformation. FST-like measures have been and continue to be widely used to describe population structure, and their characteristics and behavior are well-known. There are additional related statistics (e. g. à Ã¢â‚¬  ST (4), AMOVA, RST (20), ÃŽÂ ¸ (25)), that address other aspects of differing genetic models, unequal sample sizes, accounting for haploid genomes, mechanism of mutation and selection. D. [LR2]This is sometimes referred to as Josts D, since there are numerous other Ds related to genetics and statistics. There can be logical inconsistencies for estimating differentiation based directly on heterozygosity. Ratios of pooled subpopulation to total population diversities tend toward zero when the subpopulation diversity is high (12). Josts D is based on the effective allele number (see below). Unlike those based directly on heterozygosity, it has the property of yielding a linear response to changes in allele frequencies and is independent of subpopulation diversity. Unlike FST, GST and similar indices, Josts D does not carry information relevant to the evolutionary processes responsible for the present composition of a subpopulation. It is described by supporters and detractors as purely a measure of differentiation (26). It was never meant to do more. Whitlock provides one of the best comparisons of these 2 approaches: This (Josts) D differs from FST in a fundamental definitional way: FST measures deviations from panmixia[2], while D measures deviations from total differentiation. As a result, their denominators differ, and thus, the two indices can behave quite differently. D indicates the proportion of allelic diversity that lies among populations, while FST is proportional to the variance of allele frequency among populations. D is more related to the genetic distance between populations than to the variance in allele frequencies; it may be preferable to call D a genetic distance measure (26). There has been controversy about the use of these different types of indices. There should not be. They clearly address different questions and resolve different analytic problems. It should be recognized that the GST and Josts D yield fairly similar results when the number of populations is small and the markers have a small number of alleles. The GST and Josts D have given similar results in our own studies using microsatellites (2) and in simulation (26) with GST values slightly higher than those of Josts D. Some authors recommend calculating both GST and Josts D, in part to satisfy everyone and in part to obtain the useful information about population diversity their departure may provide. In relation to public health, most questions about parasites and vectors deal with near term events of DIVERSITY Diversity like differentiation has myriad formulations and interpretations. The simplest expression is mean heterozygosity (H). For microsatellite data this is usually high due to the intrinsically high mutation rate of these markers, and markers with higher variability are usually selected. Allelic richness (Ar) is simply a count of the number of alleles at each locus. Differences in sample size will necessarily result in differences in allele number. This is usually adjusted for by statistical methods such as rarefaction (15) to standardize sample sizes between comparison groups. The effective allele number (Ae) is also a measure of diversity, but is already adjusted for sample size. It represents the number of alleles with equal frequencies that will produce the same heterozygosity as that of the target population. The most informative measure of diversity is the effective population size (Ne), a concept also introduced by Sewell Wright. It is designed to address the essential reason that diversity is important, namely, it reflects the strength of genetic drift. Genetic drift is the effect of random transmission of alleles during reproduction to succeeding generations. When numbers of reproducing individuals are small, the genetic composition of the population of offspring can differ by chance from what is expected given the composition of the parents. If two coins are flipped, it would not be that unusual for both to come up heads. If a thousand coins are flipped, the ratio of heads to tails will always be very near the expected 50:50 ratio. Genetic drift is stronger when populations are small or reduced, and weakens the strength of adaptive selection. Like differentiation, there are several formulations for Ne that can provide different values and are designed to measure different aspects of the population. The breeding Ne is the probability of identity by descent for two alleles chosen at random. It is a retrospective assessment of population diversity. The variance Ne assesses the variance of the offsprings allele frequency, and is thus forward looking. It measures recent population changes that affect its genetic composition. Ne can represent the number of actively breeding individuals in the population or the number of individuals in an ideal population needed to reconstitute the diversity in an actual population. It is almost always less than the census population (Nc). It is a key value in conservation genetics and population genetics in general, since it reflects the history and future potential of a population. Increasing drift (decreasing Ne) tends to neutralize the force of directional selection, permits retention of del eterious mutations and hampers the ability of populations to adapt to stresses (9). Despite its importance, Ne can be difficult to estimate in wild populations due to uncertainties of the demographic, genetic and biological context (17, 24). It can be affected by sample size, overlapping generations, sampling interval, sex ratios, gene flow, age-structure, variation in family size, fluctuating population size or selection. Increasing the numbers of markers is less important than large samples for accurate estimates; as much as 10% of the Ne has been recommended [Palstra, 2008 #84]. Its interpretation can also be uncertain. Estimated Ne has been used as an aid in predicting extinction using the concept of a minimum viable population size. Some have suggested that at an Ne of 50-500 a population will experience extinction in the short- or long-run (7). Others have argued that this might occur at Ne = 5000 (14). While it is clear that lack of diversity has an impact on extinction (21), it is also clear that there cannot be a universally accepted number for the minimum viable population size (6, 23). In any case, theory suggests that there is a number defined by the amount of genetic drift below which populations are likely to go extinct on their own. The range for this number is context-specific and will require multiple species-specific studies under multiple conditions. This kind of analysis might contribute to developing a stopping rule as control measures approach elimination.         Ã‚   [1] Leptospira spp. are an exception with 2 circular chromosomes. [2] The condition where all individuals have an equal opportunity to reproduce with all other individuals [LR1]G? [LR2]Does it stand for something?

The Rise in Political Power of 17th Century England and France :: European Europe History

The Rise in Political Power of 17th Century England and France In the seventeenth century, the political power of the Parliament in England, and the Monarchy in France increased greatly. These conditions were inspired by three major changes: the aftermath of the reformation, the need for an increased governmental financing, and the reorganizing of central governments. These three points were each resolved in a different way in both England and in France. The first major point which eventually increased political power was the aftermath of the Protestant reformation. In England, after the establishment of the separate Anglican church of England there were many protestant groups left in England still in conflict. These groups all tried to push and pull parliament in their favor -- which ultimately made it so that nothing could be done. These conflicts even came to the point of bloody civil wars and suffering on both sides of the fighting. Parliament ultimately decided to stop these wars by creating religious Act of Toleration (1689) for the non-conformist protestants. For many people, this caused more unity in England and increased power. In France, the decision was made to unify the country through the establishment of a single religious authority, the Catholic Church. The king of France became the heart of this policy, which gave him control of religion when this went into effect. The next major point was the increase in need for governmental financing. In England, taxing had become under the control of Parliament. In addition to this, the cost of running a government in general had gone up and the country needed more money. Because the king didn't have as much power to tax as he pleases, the government could make a firm and accurate taxation of the people. In France, the price of government had also gone up. However, here the was a monarchy and a king with great control over the government. Using his control of the economy, a strong "Mercantilism" system was used aimed at maximizing foreign exports and reserves. The king became the center of this new power. The last major point which increased political power was the reorganizing of the central governments in both England and in France. The economic changes in this century required new relationships between the King and his subjects. In England, the parliament because of this need, grew to have power over the king and cause great toleration of people's

Are All Interpretations Possible? :: Philosophy Essays

Are All Interpretations Possible? ABSTRACT: Two fundamental criticisms made by traditional hermeneutics against philosophical hermeneutics are that the latter deny the possibility of objectively true interpretation, as well as assert that all interpretations are possible on the basis that they cannot be measured. In my paper, I argue that the first criticism is well-founded, while the second is not. I contend that interpretations can be decided according to two relational criteria: (i) which interpretation has a more comprehensive horizon; and (ii) which one is derivable from the other. 1. The birth of the philosophical hermenutics and its hermeneutical novelty. Until now it seems to be the most widespread viewpoint about the philosophical turning of hermeneutics that it was realized by Martin Heidegger in his lectures in the 1920's, and in his work: Being and Time. Let me refer to the manuscript from the 1920's that Thomas Sheehan and Theodore Kisiel found and they published in the Dilthey-Jahrbuch (Phà ¤nomenologische Interpretationen zu Aristoteles /Anzeige der hermeneutischen Situation/) (1) and refer to the volume 61. of Gesamtausgabe (Phà ¤- nomenologische Interpretationen zu Aristoteles. Einfà ¼hrung in die phà ¤nomenologische Forschung) and to the volume 63. (Ontologie /Hermeneutik der Faktizità ¤t/). Although he hardly wrote more than half a page explicitly about hermeneutics (7. §.) in Being and Time, but its work in the book is not questionable. As to the secondary grounding of my statement I refer to two competent authors'opinion. Otto Pà ¶ggeler wrote the following about Being and Time in his 1963 monograph: "Weil der Se inssinn dessen, was Husserl als das transzendentale Ich faBt, von Heidegger als faktische Existenz bestimmt wird, die in sich selbst hermeneutisch ist, wird die transzendentale Phà ¤nomenologie Husserls bei Heidegger zur hermeneutischen Phà ¤nomenologie. Die hermeneutisch vestandene  »transzendentale Erkenntnis « ist ineins Frage nach dem Seinssinn des Daseins und nach dem Sinn von Sein und damit  »ontologisch «, ErschlieBung des Seins." (2) Hans-Georg Gadamer who completed the philosophical hermeneutics that was created by Heidegger, and who is the doyen of it in our age, also stated about Being and Time that "...as a result of the existential futurality of human Dasein, the structure of historical understanding appears with its full ontological background". (3) We can not doubt that Heidegger composed the existential hermeneutics with the fundamental-ontological, philosophical intention of radicalizing Husserl's phenomenology. Let us focus our attention on what are the most important ideas — for my topic — of the young Heidegger's philosophy and its hermeneutical novelty! As we know, Heidegger considered that the fundamental question of philosophy was the question of the meaning of Being.

Profile of the Self Essay -- Decision Making Personality Essays

Profile of the Self  Ã‚   â€Å"You are requested to close the eyes.† This urging that Sigmund Freud experienced in a dream helped to unlock repressed feelings, and gave him insight into his personality. Fortunately, there are now tests available to help us to understand ourselves. Our behavior can be determined and understood by analyzing different aspects of ourselves. The four main aspects are: Decision Making, Self-Concept, Interpersonal Relationships, and Affect. Decision Making is very important.   Ã‚  Ã‚  Ã‚  Ã‚  Making decisions is really much more complicated than it seems. When I make decisions, my Id, Ego, and Superego decide my fate. Unfortunately for me, my Id wins far too often, as I often do not study enough. Other personality traits, such as my tendency to over-think and analyze too many things, also play a role. This can influence my other behaviors by making me seem cold and distant with others because I’m too busy thinking. My self-concept is also important.   Ã‚  Ã‚  Ã‚  Ã‚  Self-concept is how I view myself. This affects me in a number of ways. My self esteem and role expectations are all based off of my self-concept. My self-concept also can be a self-fulfilling prophecy. If I think that I have low self discipline, then I may subconsciously change my behavior to achieve what I think I am or should be. My real self still does not meet with my ideal self, which could potentially cause problems, or cause things to go well. My inadequacies in orderliness can never improve as long a...

Week 4 Quiz

Week 4 Quiz 1. An example of a  secessionist minority  would be a. Native Americans. b. the Amish. c. Irish Catholics. d. Italian Americans. 2. The pattern of grandchildren of immigrants increasing their interest in their ethnicity is called a. ethnic pluralism. b. the principle of third generation interest. c. ancestor worship. d. the theory of bicultural immersion.    3. What is the  ethnicity paradox? a. The emphasis on ethnic foods and ethnically associated political associations instead of deeper ties. b. The maintenance of one’s ethnic ties in a way that can assist with one’s assimilation in larger society. c. The belief that one’s own culture is not as good as the dominant culture in society. d. The social acceptance of prejudice toward White ethnics.4. Members of which White ethnic group were placed in an internment camp in Montana during WWII? a. Japanese b. Italians c. Jews d. Irish5. Which two White ethnic groups found, upon immigrating to America, that their shared religion did not trump their cultural differences, to such a degree that clergy were imported from Europe?a. Germans and Irish b. Irish and Italians c. Jews and Italians d. Germans and Poles6. Which of the following would NOT be considered a White ethnic group? a. Jews b. Italians c. Chinese d. Swedes7. The 1962 Supreme Court decision  Engel v. Vitaleà ‚  ruled which of the following unconstitutional? a. Christmas displays in public schools b. school prayer c. certain religious activities in schools d. teaching creationism8. A large, organized religion that is not officially linked with the state or government is known as   Ã‚  Ã‚  Ã‚  Ã‚   a. n ecclesia. b. a denomination. c. a cult. d. a sect.9. Which of the following faiths has the largest membership in the United States? a. the Roman Catholic Church b. Jewish congregations c. Southern Baptist Convention d. the United Methodist Church10. Civil religion  describes a. governments run by the church. b. belief systems incorporating many religions, but no single one. c. courts restricting religious freedom. d. New Age faiths